. El boletín biológica. La Biología, Zoología; biología; biología marina. e f g FIGURA 4. Localización histoquímica de la actividad de AChE en H. roretzi todo y cuarto de embrión de diversas edades, (a, b, c) toda Dechorionated embriones después de 22 h (a), (b) de 28 h y 32 h (c) de desarrollo, respectivamente. En (a) las células musculares de la región de punta de caudal no producen dolor, pero en (b) y (c) muestran la actividad de la enzima. Punta de flecha (B) indica la faringe con AChE actividad primordial y puntas de flecha en (c) indicar pequeñas manchas con la actividad de la enzima, (d) B4.1 trimestre embriones con actividad AChE (30 h de

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. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. e f g FIGURE 4. Histochemical localization of AChE activity in H. roretzi whole and quarter embryo of various ages, (a, b, c) Dechorionated whole embryos after 22 h (a), 28 h (b), and 32 h (c) of development, respectively. In (a) muscle cells of the caudal tip region do not produce AChE, but in (b) and (c) they show the enzyme activity. Arrowhead in (b) indicates the primordial pharynx with AChE activity and arrowheads in (c) indicate small spots with the enzyme activity, (d) B4.1 quarter embryos with AChE activity (30 h of development), (e) b4.2 quarter embryo at 30 h of development. A few cells show the enzyme activity, (f) A4.1 quarter embryo with the enzyme activity (30 h of development), (g) a4.2 quarter embryos developing AChE activity in a few cells (46 h of development). Scale bar. 100 morphogenesis. At this time, however, muscle cells of the caudal tip of the tail did not show the enzyme activity; AChE activity in this region first appeared in 24-h embryos (Fig. 4b). Like Ciona embryos, normal Halocynthia embryos also produced AChE in tissues other than the tail muscle cells. AChE activity was noticed in the region between the sensory vesicle and the papillae in 24-h embryos (Fig. 4b). This structure is in the region of the primordial pharynx. In addition, 32-h embryos produced AChE in several other small spots near both dorsal and ventral sides of the primordial pharynx region (Fig. 4c). Acetylcholinesterase development in quarter embryos In this report, partial embryos derived from isolated a4.2-, b4.2-, A4.1-, and B4.1-blastomere pairs are designated for convenience as a4.2, b4.2, A4.1, and B4.1 quarter embryos, respectively. AChE activity in these quarter embryos is summarized in Table I (C. intestinal is) and in Table II (H. roretzi). The B4.1 quarter embryos. According to recent cell lineage study (Nishida and Satoh, 1983), among the 36 (C. intestinalis) or 42 (H. roretzi) muscle ce

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• bookauthorlilliefrankrat
• booksubjectbiology
• librosubjetzoología